Sentence examples for repeated integrated from inspiring English sources

This discrepancy may reflect differences in the genetic requirement for recombination between directly repeated integrated DNA sequences which involves single-strand annealing versus that for gene conversion [64], [65] as assayed in this study by PCR-mediated restoration of HIS3 following transformation.

For repeated integrated urban wastewater system simulations that do not require repeated training of the ANN, the ANN reduces simulation time by a factor of 1300 compared to the mechanistic model.

The HT1080 cell with lacO repeats integrated in chromosome region 11q13 was described previously [16].

To directly observe how the sister centromeres were segregating in individual dis3-54 andoublele dis3-54 Δmad2 mutant cells, we crossed these mutants with a strain that expresses LacI-GFP and contains lacO repeats integrated at the lys1 locus that is close to the centromere of chromosome 1 (cen1-GFP; [18]).

These papers used likelihood to estimate repeat counts, without considering other problems, such as how exactly are repeats integrated within scaffolds.

As a more direct test of the ability of Sgo1 to bring condensin to chromosomes, we produced a Sgo1-GFP-TetR fusion protein in cells carrying tetO repeats integrated on a chromosomal arm.

We produced Scc4 fused to bacterial LacI in wild-type and chl4Δ strains carrying lacO repeats integrated adjacent (∼500 bp) to the centromere on chromosome IV (CEN4; Figures 1A and 1D).

As a means to directly examine the status of meiotic homologous pairing in vivo, we built a strain, AV221, that carries a transgene insertion (meIs1 ) expressing GFP-LacI using the germline-specific pie-1 promoter (Praitis et al. 2001; Yuen et al. 2011) and a large array of lacO repeats integrated into a single genomic locus on chromosome III (meIs4 ).

This hypo-recombination defect in gene conversion for some (tho2Δ, mft1Δ, thp2Δ) but not all (hpr1Δ; [40]) mutated members of the THO complex, is in contrast to the hyper-rec phenotypes described for THO defects when assayed by loss of stability in directly repeated sequences integrated at LEU2 [49].

The efficient mechanism for the rapid extension and controlled termination of RNA primers by Pol α uncovered here seems ideally suited for the frequently repeated, highly integrated priming events taking place on the lagging strand template.

Similarly, the same procedures can be repeated to integrate the genes of Γ1 and the associated links to Γ2.