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The 3D bias for the modified calibration method using the new calibration object was 0.032 ± 0.006 mm, the 3D repeatability standard deviation was 0.015 mm, and the 3D repeatability limit was 0.042 mm.
The means of log10 viable cell densities (± the repeatability standard deviation of log densities) were 7.2 (± 0.31) for the bulk water (density in units of cfu ml− 1), 5.3 (± 0.56) for the coupons (density in units of cfu cm− 2), and 6.6 (± 0.50) for the filters (density in units of cfu cm− 2).
If the variation in the responses corresponded to approximately twice the repeatability standard deviation, individual values for parcentage label claim (%LC) response may lie outside the specified limits; this implies the data are not centered between the specified limits, and that this term plus the sampling error affects the AV value.
scalc represents the maximal acceptable value for repeatability standard deviation (s x ).
The repeatability standard deviation (SD) of the un-patterned sample, which quantifies the repeatability of the methods based on control variances, is the square root of the sum of the among-experiment variance and the within-experiment variance divided by the number of replicates per experiment [39].
From Table 4, it can be seen that the repeatability standard deviation of our routine method is usually below the ENGL criterion of 25%.
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Using the ten sequential sets of measurements, the repeatability standard deviations were found to be 0.10, 0.11 and 0.06 cm for x, y and z, respectively.
Precision estimates were calculated as repeatability standard deviations of the Ct (threshold cycle number) values from the amplification of each plasmid dilution.
In addition, the mean value and standard deviations of these repeatability standard deviations were also presented in Table 1 to provide a measure of the variance of these estimates.
Good repeatabilities (standard deviations < 10%%) were achieved for the vast majority (83%%) of all cases.
Furthermore it had a good reproducibility and repeatability with standard deviation of 9.4% and 8.0%, respectively.
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