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Our results demonstrate the existence of potent pQ variants of Notch and the need for long read genotyping of key repeat variables underlying gene regulatory networks.
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repeat variable diresidues.
RTLs have a unique DNA-binding architecture and are enriched in repeat variable di-residues (RVDs), which determine repeat DNA-binding specificities.
Each TALE repeat consists of 33 to 35, typically 34, highly conserved amino acids except for the two at positions 12 and 13, which are named RVDs for repeat variable diresidues (RVDs).
The repeat variable di-residues (RVD) selective for the different deoxyribonucleotides are color-coded (single letter amino acid notation).
The repeat variable diresidues (RVD) selective for the different deoxynucleotides are color-coded (single letter amino acid notation).
Each repeat is highly conserved with the exception of two amino residues, termed the repeat variable diresidues (RVDs), which are responsible for binding specificity to each individual nucleotide.
A pair of residues at the 12th and 13th position of each repeat region determines the nucleotide specificity and are referred to as the repeat variable diresidue (RVD).
Specificity of DNA-binding of TALENs is mediated by two amino acids in each of the individual repeat domains, the so-called repeat variable di-residue (RVD).
Each repeat interacts with a single nucleotide, the specificity of which is determined by two amino acids, the so-called repeat variable di-residues (RVDs).
These "RipTALs" have a similar repeat variable diresidue domain and several RipTAL RVDs appear to have affinity for specific letters of DNA.
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