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However, a crucial factor in deciding the optimal age (or ages) involves obtaining a balance between identifying the majority of cases, and not having to repeat the screening many times.
In other words, before ordering further laboratory tests to investigate the prolongation of PT and/or APTT, clinicians should consider to repeat the screening tests to exclude false results.
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However, two factors led us to not simply repeat the screen.
All variants found in the screening have been confirmed by either DNA sequencing or repeating the screening by respective method (SSCP, CSGE, RFLP) for the genomic DNA sample.
Guidelines from the American Cancer Society and US Multi-Society Task FonColorectalreCancerancerecommendnd repeating the screening colonoscopy before planning a long-term surveillance program if the colon is not adequately prepared [ 19].
If so, these false-negatives could potentially be identified by repeating the screening in multiple cell lines that have different miRNA profiles, a point that is now made in the Discussion section.
In order to estimate the false discovery rate (FDR), we repeated the screen with shuffled alignments as described in [ 33].
To assess whether these histone H3 sequences are conserved between mouse and human, we repeated the screen on the human genome (hg19).
Depending on the pathogen and library, we independently repeated the screens one to six times as replicates (see Additional file 1: Table S1).
To exclude the possibility of off-target effects in the 19 hits, we repeated the screen using the individual siRNAs that were used in each siRNA pool in the primary screen.
To reduce the possibility that the NAMPT effect identified in the screen was an off-target effect, we repeated the screen assay using three different siRNA species, each targeting NAMPT.
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