The failure caused the total loss of the crossing, and the necessity to repeat the entire process in another, less favorable location.
Moreover, the fixation protocol we employed preserved the cells in suspension for future experiments, without the necessity to repeat the cell synchronization and BrdU treatment during each experiment.
They concluded that a single negative biopsy for malignancy can be regarded as a true negative with no necessity to repeat the biopsy.
Furthermore, questions remain about the number of doses needed to obtain an optimal and lasting immune response and the necessity of repeating the vaccination.
The patient and referring clinician should be advised as to the possible need for repeat treatment and the necessity of appropriate follow-up to detect and treat early tumour re-growth and so control the liver tumour load.
Recurrent infections are of particular concern because of the repeated morbidity and necessity for frequent administration of antibiotics with the possible adverse consequences of selection of antimicrobial-resistant strains.
The amplification protocols utilized here allow buccal cell samples to be used in repeated measures experiments removing the necessity to sample more than once to obtain sufficient template for a single microarray.
The necessity to repeat scans in case of equipment that is out of order will directly lead to additional costs for radiopharmaceuticals and personnel.
However, in our present study, several patients experienced disease relapse after 6 months, indicating the necessity to repeat MSCT after 6 months.
The necessity to repeat phrap on large datasets probably merges distinct but similar sequences excessively, leading to a loss of microsatellites but also a canceling loss of "nonredundant" sequence to host them.
