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However it appears that a typical phylogenetic analysis of sequence data involves five distinct steps, one of which is 'alignment trimming' [ 20] choosing of core areas of genes without extensive repeat supported both the phylogenic analysis and primer design.
Second, RT-PCR with overlapping primers spanning the ∼3.4 kb from the DBE-T start to the end of the DBE of the first D4Z4 repeat supported a single AZA+TSA inducible long RNA.
Disruption of either the putative stems or loops of the repeat A abrogated silencing, and mutations of the first palindrome to enforce pairing within a repeat, or between the first and second repeat, supported models that the first palindrome forms a hairpin.
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Figure 3B shows the frequency distributions of the (AAAATAGAAT) repeat, supporting the presence of long occurrences of the STR in SCA31 and the absence of long occurrences of length >60 bp in the other control samples.
The results from genotyping multi-allelic short tandem repeats supported founder effect in the identical mutations in the 3 unrelated patients.
The observation that mRNA levels are reduced in HD mouse models with super-long CAG repeats supports this suggestion.
There is one region on CHR4 in the E. cymbalariae genome that carries the rDNA repeats (supporting information, Figure S3).
The discovery of two novel retrotransposon derived tandem repeats supports the conclusions from earlier studies that retrotransposons can give rise to tandem repeats in eukaryotic genomes.
The highly similar iterated repeat array of our complete Argiope argentata AcSp1 gene combined with sequence conservation of functionally important regions of individual repeats supports a hypothesis of concerted evolution and functional constraints acting together to homogenize Aar_ AcSp1 repeats.
The observed dominant passive diameter approximates the distance of adjacent hydrophobic clusters of FG repeats, supporting the model that the barrier is made of FG repeat domains cross-linked with a spacing of an FG repeat unit length.
In addition, the significant positive correlation between the number of indels and repeats supports the hypothesis that repeat sequences may promote the creation of indels (supplementary table S1, Supplementary Material online).
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