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Input into the script requires the sequence file in fasta format, the minimum and maximum repeat length to find, and a minimum repeat periodicity.
Results: The five spectra of the candidate sequences coding for structural repeats, obtained by Discrete Fourier Transform (DFT), show common features allowing determination of repeat periodicity with excellent results.
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Repetita [25] was used to predict repeat periodicities.
Since the NgR protein has a longer LRR-CT and 8 repeats, the analysis of repeat periodicities with Repetita was performed to identify the correct number of LRR repeats in LGI1.
The repeated periodicity in out-of-plane direction (z axis) in structures, called the Talbot distance (Z T), prepared by PnP can be calculated by using the following formula [41].
and a maximum repeat period of 3. Identified repeats were further filtered to remove all repeats with a period of one or two; period-one repeats have multiple periodicities, but were here considered to be mono-nucleotide repeats and were excluded from further consideration.
These assemblies form extensive chains with typical repeat distances (periodicities) of 56 nm [26,45].
This experiment was then repeated for approximate periodicity, for which we defined Gaussian distributed periods with expected value of 10 and standard deviation σ ∈ [0.1, 3].
Comparison of the two genomes revealed that both predicted proteins contain a central portion of repeats with a periodicity of 28 residues and that the GT1 protein is predicted to contain an additional three repeats (data not shown).
The resulting variants were filtered for overlap with elements annotated as simple repeats with a periodicity <9 in the UCSC Genome Browser [ 42, 43], homopolymer runs >8 bp (plus 1 bp either end), dinucleotide repeat runs >14 bp (plus 1 bp either end) and regions with an average mapping quality score <40.
S-layer proteins show the ability to reassemble into two-dimensional arrays on solid surfaces and their functional groups, such as carboxylic groups, are repeated with the periodicity of the lattice, allowing for immobilization of other (bio molecules.
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