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At pH 9.0, there was little bias in orientation for the few insertions that occurred in the repeat (Figure 3).
Finally, the interruptions were largely clustered within the distal third of the repeat (Figure 1), enabling us to determine if these interruptions affected transposition within the repeat.
At pH 9.0, where the acid stabilized YRY triplex formation is discouraged, only 14% of 96 insertions occurred within the repeat (Figure 2).
ISSsp1 which had imperfect inverted repeats (32-bp in length, one mismatch) was flanked by two copies of 8-bp direct repeat (Figure 2B).
The nrdH gene encoding glutaredoxin had a 14 bp deletion in its potential promoter region compared to that of Rv3053c in H37Rv, where it forms a duplicated tandem repeat (Figure S1).
To determine whether the insertion of a transposon would inhibit triplex formation, we analyzed the electrophoretic mobility of plasmids containing a Tn5 transposon at different areas along the repeat (Figure 4).
Similar(44)
His loops are being voiced neither by humans — who repeat figures in a way that involves a fairly high level of variation — nor by digital devices like samplers and software programs, which come close to no variation at all.
The music, by Arvo Pärt, is extremely spare: a rudimentary violin melody set over a repeated figure on the piano, a few chords.
Michael Byron's string quartet "Awakening at the Inn of the Birds" (2001) built an attractive, rhythmically dense fabric from a robust, repeating figure.
Mr. Curtin's figure, based on scrutiny of shipping contracts and port data, was substantially less than the widely repeated figure of 20 million, which, he showed, was an extrapolation from now-lost records pertaining to Jamaica, and the figure of 15 million used by W. E. B. Du Bois, who repeated a figure arrived at by Edward Dunbar, a 19th-century abolitionist.
Ebbing and flowing in waves, the music ended with an obsessively repeated figure as if a phonograph needle were stuck in a groove.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com