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GC content, repeat distribution and gene density in a 1-Mb sliding window are shown at 100-kb intervals.
This indicates that chromosome level organisation has been shaped by events which are largely insensitive to repeat distribution.
The repeat distribution for isoleucine tails off very quickly and phenylalanine is under-represented amongst the proportion of repeats.
The ∼2000 nt long sequences were sorted after repeat distribution patterns, for their visualization customized Genbank formatted feature files were generated and imported into Vector NTI (Invitrogen).
This might suggest a relationship between the asparagine repeat distribution and the parasite host, and that glutamine repeats are adaptive to primate parasites.
We analyzed the repeat distribution and found that transposons are virtually absent from transcribed regions but that they can accumulate within the clusters at untranscribed regions.
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Overall, repetitive sequences amounted to 7.5% of the T. parvula genome based on similarity searches against genomic repeat databases and de novo clustering of repetitive sequences (Supplementary Tables 2,3). Figure 1a and b show repeat distributions in combination with overall sequence alignment comparisons using Circos plots.
This shows that the significance of features in the amino acid repeat distributions is different between short and long repeats.
The variability in the repeat distributions between lineages (supplementary table S1) indicates that repeat expansion is not a stochastic process and that selection plays a role in some amino acid repeat distributions.
Table 3 shows the chi-squared tests for the asparagine repeat distributions across a number of eukaryotic organisms.
As word length is restricted to four this model includes bias in the shorter length repeat distributions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com