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We then dissolved the proteins in an aqueous buffer at a concentration of ≈200 mg/ml, which approximates the expected FG repeat concentration in authentic NPCs.
Having established that our model films match the thickness, the FG repeat concentration and the 'end on' mode of chain attachment that are pertinent to NPCs, we considered how NTRs interact with such FG repeat meshworks.
In other words, NTRs not only become enclosed by the barrier but also participate in barrier function; they hinder inert material from traversing NPCs and they lower the FG repeat concentration required for proper barrier function.
However, even then the question still remains if the resulting protein-rich phase has an appropriate FG repeat concentration and structure to function as a barrier with NPC-like permselectivity.
The FG repeat concentration is also comparable to macroscopic, in vitro-assembled FG repeat hydrogels that were shown recently to exhibit a selectivity of transport similar to that of intact NPCs (Frey & Görlich, 2007, 2009).
Similar(55)
In the present example, each single dataset displays changes over time that show few repeated concentration values (see Figure 6(c)).
Heptose 1 is typically isolated as a hygroscopic syrup; 5b however, a solid foam could be obtained by repeated concentration from iPrOH.
Within-group changes suggested a decrease in the repeated concentrations of oleic and linoleic acids (p = 0.1).
Buffer salts were removed from the polysaccharide samples by repeated (6×) concentration in an Amicon concentrator (1 kDa cut-off PLAC membrane) and dilution with a 10-fold volume of distilled H2O.
According to previous studies [34, 41], the possibility of sample carry over from repeat pre-concentration steps could cause significant concerns in on-line SPE methods.
The recovered lignin does not include that lost during repeated NF concentration and dilution in the flat membrane cell used in this study.
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