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Taking advantage of these strategies, WDRR could effectively reduce the false positive rate and accurately identify more remote homologous WD40 repeats with precise repeat boundaries.
This step is crucial to ensure the precise definition of repeat boundaries, one of the main challenges in the de novo detection of TEs.
Manual refinement consisted in a method similar to ABRA [35] and Kajava's method [36], with knowledge about the approximate location and number of repeats serving to identify the true repeat boundaries.
Initially, repeat boundaries were determined using RepeatMasker.
The alignment was then inspected for structural mutations and adjusted manually to preserve tandem repeat boundaries and identify inversions.
The alignment was then inspected for structural mutations and adjusted manually to preserve tandem repeat boundaries and to identify inversions.
Similar(46)
Minimus instead uses a very conservative approach where, if a repeat boundary is encountered, it stops extending the contig.
Phrap's greedy strategy cannot always handle long-range genome structures and when a repeat boundary is found it can be fooled by false positive overlaps.
Each BOG path intersection can signal a genomic repeat boundary or represent noise.
Interestingly, 5% of the single base pair substitutions might represent double-slippage events that occurred at the junction of immediately adjacent repeats, resulting in a shift in the repeat boundary.
It would be wrong to ignore all length=1 intruders; a valid read that spans a genomic repeat boundary can have no overlaps at its non-repetitive end due to random low coverage.
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