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When a specimen contained five or more HPV types in the initial assay, it was rare to identify all of the HPV types in the repeat assay.
The linoleic acid sensitivity of these mutants was reconfirmed, proving the phenotype was transposon-linked, by repeat assay using serial dilutions of the mutant strains onto BHI agar containing millimolar concentrations of linoleic acid.
We randomly chose 5% of the samples and obtained 100% concordance on the repeat assay.
The identities of all cell lines were confirmed by a short tandem repeat assay (Masters et al, 2001).
For diagnosis, a positive test result [≥6.5% (48 mmol/mol)] should be confirmed with a repeat assay.
In the repeat assay, we also detected increased GRO signal in G2B-10A conditioned medium compared to R2-T1AS medium.
Similar(44)
The activity of each compound was confirmed in repeat assays using either the Bodipy-C16 or PED-6 lipid.
For laboratories that did return data from repeat assays, the IC50s were generally within a 2 4 fold range.
Samples were maintained under laboratory codes for all assays at all three sites, including any repeat assays required to address inter-laboratory discordance.
Selection was based on the availability of sufficient serum for repeat assays and the known presence of anti-GAD65 in the serum.
For clinical samples, repeat assays were performed for randomly selected samples.
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