The intestinal epithelium is an incredibly large mucosal surface with an extreme self-renewing capacity.
Most importantly, melanoma cells that survived parthenolide treatment lost their self-renewing capacity.
CD146 is a surface marker of pericytes and expressed on a population of bone marrow derived subendothelial cells for which true self-renewing capacity was described [ 37].
Like in breast cancer [ 16] or glioma [ 21], radiation enriches for these cells by selectively killing the more radiosensitive population with high proteasome activity and lower self-renewing capacity.
Like other cell types with self-renewing capacity, keratocytes can form spheres in culture.
Compared with adherent cells, the floating tumorsphere cells had more self-renewing capacity and chemoresistance.
These data suggested that the self-renewing capacity of tumorsphere cells was significantly higher than that of adherent cells.
The self-renewing capacity of these tumor spheroids was assessed using the method published by Ghods et al [19].
For observing the self-renewing capacity further, soft agar assay was used to determine the colonies formation of tumorsphere cells and adherent cells under anchorage-independent conditions.
The self-renewing capacity of these tumorspheres was assessed by dissociating them into single cell and growing at a clonal density of 1,000 cells per milliliter.
Culturing hNSCs in FPN medium significantly reduced their growth rate, still maintaining their self-renewing capacity for at least 5 subculturing passages (Fig. 4A).
