Recent reports convincingly demonstrated the implication of p63 in epithelial stem cell renewal potential, as the absence of p63 was shown to induce a loss of proliferative potential of epidermal [5], [6], corneal [7] or thymic epithelial cells [6], [8].
To further investigate the self renewal potential, spheroids from H1650-ER1 cells were dissociated into single cells and cultured under non adherent conditions for 5 generations.
Several groups have shown that undifferentiated Spga of the first wave of spermatogenesis comprise a large fraction of cells with stem cell characteristics and self renewal potential [ 10- 13].
Breast tumor initiating cells (BT-ICs) are functionally defined by their unlimited renewal potential and ability to reproduce tumor heterogeneity, attracting attention as therapeutic targets [ 1, 2].
The use of terminally differentiated cells, as in the case of autologous chondrocyte implantation, is compared with the advantages/disadvantages of using more undifferentiated cell types, such as stem cells or early mesenchymal progenitors that retain multi-lineage and self-renewal potentials.
Neuronal stem cells are immature cells with self-renewal potentials that are able to differentiate into neurons, astrocytes and oligodendrocytes [1] [2].
Drosophila intestinal stem cells (ISCs) exhibit self-renewal potentials, maintain tissue homeostasis, and serve as an excellent model for studying cell growth and regeneration.
Therefore, cancer stem cells arise from cancer-initiating cells and are responsible for tumor recurrence (that is, proliferation and self-renewal potentials) and the tumor heterogeneity (that is, multi-lineage differentiation potential).
These observations strongly support a role for mTORC1 in regulating the self-renewal potential of resting-zone stem cells.
The mammalian target of rapamycin complex 1 (mTORC1) signalling pathway maintains the self-renewal potential of resting-zone stem cells.
ANSCs treated by SDNSF also retain self-renewal potential and multipotency in the absence of FGF-2.
