When isolated and grown in culture, CD24+CD133+ PECs exhibited high colony-forming capacity and self-renewal potential [85].
Therefore, ALDH expression may be associated with increased self-renewal potential in B-ALL.
MSCs show properties shared by embryonic stem cells; they have the self-renewal potential and can differentiate into several cell lineages including osteoblasts, chondrocytes, adipocytes and myoblasts [8].
Since primary FL transplant cannot measure true HSC self-renewal potential, we collected BM cells from primary hosts and performed BM transplant into lethally-irradiated secondary hosts.
The inhibition of pathway activity primarily impacted highly clonogenic B-ALL cells expressing aldehyde dehydrogenase (ALDH) by limiting their self-renewal potential both in vitro and in vivo.
Tumorsphere culture has been widely used to assess the self-renewal potential of stem cells and cancer stem cells [5], [14], [32], [39].
Upon differentiation, HSCs irreversibly enter progenitor cell stages characterized by extensive proliferation at the expense of their self-renewal potential [5].
These tumor initiating B cells also share functional features with normal stem cells such as drug resistance and self-renewal potential.
Moreover, ALDH+ cells displayed increased clonogenic expansion during serial passage compared to ALDHneg cells suggesting that they had increased self-renewal potential.
Shp2-deficient mES cells displayed dramatically impaired capacity of differentiation into all three germ layer cell lineages, accompanied by improved self-renewal potential.
