To determine whether AspM-CreERT2 long term descendants that are detected in the adult SVZ, display features of neural progenitor cells, we tested their multipotency and self renewal capability by using standard clonal colony formation assay [44], [45].
From an ecological perspective, natural mortality plays a fundamental role in the year-to-year dynamics of a population and its long-term renewal capability.
Reversing cancer stemness, together with conventional chemotherapy, could provide an ideal approach for prevent cancer recurrence and metastasis by eradicating both the bulk tumor cells and the cancer stem cells with self renewal capability.
The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential.
It is now well established that human pancreatic ductal adenocarcinoma (PDAC) contains a subset of cells with self-renewal capabilities and subsequent exclusive in vivo tumorigenic capacity as assessed by limiting dilution tumorigenic transplantation assays into immunodeficient mice.
Only part of the cultures containing oncospheres exhibited self-renewal capabilities (Figure 1, left panel).
IPCs have been suggested to have self-renewal capabilities [14], [22] and one could argue Fig. 6H N illustrates one such incidence.
Unlike in normal development, however, neoplastic progenitor cell populations can gain self-renewal capabilities, thereby also fulfilling the definition of a CSC [2], [3].
