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We carried out immunohistochemistry experiments and visualized the sections with an epifluorescent microscope (Zeiss AxioImager) or confocal microscope (Olympus confocal FV1000) and 3D rendering performed using Volocity software.
Acquired z-stacks were background-subtracted, and 3-D isosurface rendering performed using Bitplane IMARIS suite version 7.1 x 64 software (Bitplane Inc. Saint Paul, MN).
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The evaluation to understand how a render performs over time is a complex activity, not only due to the multiple factors concerning its exposition, but also to the difficulty in assessing the in-service behaviour.
In these circumstances, the combined capsular and bone deficiency would render performing a Bankart procedure difficult and unreliable [ 7, 10, 20, 31, 42, 47, 49, 51].
Three-dimensional rendering was performed in ImageJ/Fiji (http://rsb.info.nih.gov/ij/).
Additionally, for composite elements (a captioned picture for instance, see Sect. 4.4), the rendering is performed recursively inside the element's corresponding placeholder on the page.
Data rendering was performed using SAS 9.1 and statistical analyses were performed using Stata 11.0.
Iso-surface rendering was performed on the combined Z-stack images using Imaris imaging software.
Surface rendering was performed by gray level thresholding using the Chimera software (http://www.cgl.ucsf.edu/chimera) after having low pass filtered the tomograms to 1/10 nm−1/10
Image reconstruction and multi-dimensional rendering were performed with Imaris (Bitplane), while tracking and measuring aspects of cellular motility were performed with Pic Viewer Software (John Dempster, University of Strathclyde) [10], [26].
Surface rendering was performed independently on each channel to obtain the geometric centers of the nuclei (blue channel, DAPI) and the chromosome territories (red channel, Spectrum orange and green channel, Rhodamine Green), respectively (Figure 3).
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