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Such cells, when lyophilized without any lyo/cryoprotectant, were rendered non-viable but fully retained cadmium precipitation ability.
Recently, our group has demonstrated that somatic sheep cells rendered non-viable by heat treatment retain the potential to generate normal offspring following nuclear transplantation [10].
Tests for rapid diagnostic work involving smears and molecular methods do not require CL3 practices after the organism has been rendered non-viable.
After 24 h of caspase activation in revC3 cells, caspase activity was reduced to baseline, which when correlated with cell viability) was consistent with ∼50% of these cells being rendered non-viable (P⩽0.03 versus all other groups).
Liquid wastes may be autoclaved at 121°C for 15-20 minuthe in the case of small volumes; up to 60 minutes for volumes up to 10 L. If a suitably sized autoclave is not available or large volumes (>10 L) of spent growth medium are to be disposed of, a chemical oxidant should be used to ensure that all cyanobacterial cells are rendered non-viable.
Metabolic reactions play a fundamental role in sustaining cell growth through the import of nutrients from the environment and their conversion into molecules rendering a viable organism.
This mitochondrial potential-sensitive dye renders the viable cells fluorescent (see Figure 3(a), untreated controls), because of their high transmembrane potential (lower-right quadrant), while its uptake is reduced in early and late apoptotic cells.
For the identification of residual (incompletely ablated) tumour, dynamic Gd enhancement may play a role, and in particular a double-contrast method where iron-oxide particles are administered to render the viable parenchyma hypo-intense prior to dynamic Gd-enhanced imaging has been proposed to identify incompletely ablated tumour regions[ 28 ].
While cells in the absence of revC3) or in presence or absence of revC3 C/A) expression displayed the same growth kinetics in vitro as the parental cell line, dox induction of revC3 rendered cells non-viable within 24 h).
