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Priming substances like cytokines or LPS render quiescent neutrophils more susceptible to secondary stimuli such as fMLP, bacterial exotoxins, or cell cell contacts without activating them by themselves [ 32].
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For inhibitors treatment, hGF were rendered quiescent by 24-hour incubation in DMEM with 0.5% FBS.
The cells were rendered quiescent by incubating in serum-free media for 36 hours.
Line1 cells were rendered quiescent by serum starvation and treated with 1 µM nicotine for 48 h.
Eighteen hours post transfection, the cells were rendered quiescent for 36 hours by incubation in serum-free media.
Panc-1 cells have been rendered quiescent by 72 h serum deprivation (control group) and were then treated with KGF or 10% FBS for 24 h.
The NHBEs and SAECs were rendered quiescent in basal media containing ¼ the amount (v/v) of growth factors for 24 hours (cit).
The cells were rendered quiescent 24 hours later by serum starvation for a period lasting a further 24 hours, and then stimulated by adding medium containing serum.
Line1 cells were plated in poly-D-lysine coated chamber slides at a density of 10,000 cells per well and rendered quiescent by serum starvation for 72 h.
When indicated, C2C12 and Myf5-LacZ myoblasts were rendered quiescent through 72 h of culturing in a methocellulose supplemented medium that prevented cell adhesion as described previously [27].
For synchronization, human fibroblasts were rendered quiescent by serum deprivation for 48 hr and then stimulated to reenter the cell cycle by the addition of serum to a final concentration of 10%.
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