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Such cells, when lyophilized without any lyo/cryoprotectant, were rendered non-viable but fully retained cadmium precipitation ability.
Recently, our group has demonstrated that somatic sheep cells rendered non-viable by heat treatment retain the potential to generate normal offspring following nuclear transplantation [10].
Tests for rapid diagnostic work involving smears and molecular methods do not require CL3 practices after the organism has been rendered non-viable.
After 24 h of caspase activation in revC3 cells, caspase activity was reduced to baseline, which when correlated with cell viability) was consistent with ∼50% of these cells being rendered non-viable (P⩽0.03 versus all other groups).
Liquid wastes may be autoclaved at 121°C for 15-20 minuthe in the case of small volumes; up to 60 minutes for volumes up to 10 L. If a suitably sized autoclave is not available or large volumes (>10 L) of spent growth medium are to be disposed of, a chemical oxidant should be used to ensure that all cyanobacterial cells are rendered non-viable.
Active ingredients in a number of the cleaning agents, wipes and tissues tested were able to target the influenza envelope and render the virions non viable.
Events off the field were to overshadow the team's performances as the club faced financial difficulties severe enough to render them non-viable.
Wild-type C. gattii cap59Δ mutant cells were treated with sodium azide (2 mM), followed by incubation at 65°C for 30 minutes to render them non-viable; these cells were used in experiments that compared effects of viable and non-viable cells.
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