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Interestingly, a calcium transient created by the calcium uncaging technique, or indeed from the native intracellular calcium oscillations, can re-sensitize an adaptive cell and render it responsive, for another short period of time, to substrate ECM guidance.
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Alternately, evolutionary events (point mutations, rearrangements, further lateral transfers in the same region) subsequent to acquisition of a gene could render it O2-responsive.
During capacitation, major changes take place in the sperm plasma membrane so as to render it fusogenic and responsive to zona pellucida glycoproteins.
Alternatively, the mutations affect the structure of the promoter rendering it less responsive to transcriptional factors.
Make It Responsive.
The Anx-A1 system in undifferentiated myelomonocytic U937 cells does not respond to glucocorticoids and it is necessary to pre-treat the cells with low concentrations of PMA to render them responsive [43].
This will partially deactivate the L-type Ca2+ channels due to the steep voltage-dependence of their gating behavior in the physiological range of membrane potentials (–60 to –30 mV), and eventually render them responsive towards the effects of hypoxia.
To date, various signalling and regulatory proteins have been modified by domain combination, swapping or evolution to render them responsive to novel input signals or able to act on non-cognate target substrates.
Given the transactivating effects of GATA transcription factors on pRL-TK and pRL-SV40, it is likely that the presence of a consensus GATA binding site close to the Rluc gene in other Rluc expressing plasmids may also render them responsive to transactivation by GATA transcription factors.
It's a vision that renders government less responsive and less responsible to its citizens.
The positively charged cationic hydrogel microparticles were embedded/dispersed into another hydrogel matrix to render responsive behavior to a non-responsive matrix.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com