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Original sequences were generated by removing vector and E. coli DNA sequences using Cross-match [ 26].
Each sequence was edited manually by removing vector sequences and the ambiguous reads.
The sequences were processed by removing vector sequences with Vector NTI Advance™ 10 program.
Raw reads were cleaned by removing Vector (Adapter/Primer) contaminated reads.
Sequences were processed by removing vector, adaptors and E. coli DNA sequences using CrossMatch [ 71].
Original sequences were processed by removing vector, adaptors and E. coli DNA sequences using CrossMatch [ 48].
Similar(43)
Before assembling the reads, all were trimmed by removing vector-specific sequences.
ESTs larger than 100 bp length and Q20 quality after removing vectors, adapters, and ploy-A tails were performed to generate a unigene set using Cap3 [ 56] with the minimum overlapping length parameters >45 bp and overlapping identity percentage >90%.
Sequences of the respective clone libraries were trimmed to remove vector and primer sequences using MEGA6.0 software (Tamura et al. 2013).
This plasmid was linearized with AatII and AseI to remove vector sequences and purified.
The cDNA sequences were trimmed to remove vector sequences that may bias the integrity of homology-based searches using the NCBI UniVec database (ftp://ftp.ncbi.nih.gov/pub/UniVec/).
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