Sentence examples for removing the solution from inspiring English sources

Cells were then washed with PBS and 100 µl of 5 x TdT Equilibrium Buffer was added (1∶5 TdT: H2O) and left to incubate for 10 min. After removing the solution, 30 µl of 1∶20 Fluorescein Labelling Reaction Mix: TdT Enzyme was added and incubated for 1 hour at 37°C.

After removing the solution gel pieces were alkylated with 100 µl 55 mM iodoacetamide in 40 mM NH4HCO3 for 30 min at 25°C in the dark, followed by three alternating washing steps each with 150 µl of 40 mM NH4HCO3 and ethanol for 5 min at 37°C.

After removing the solution, 30 μL of 50 mM ammonium bicarbonate was added.

After removing the solution, the gel spots were incubated with 200 mM ammonium bicarbonate for 20 min.

After removing the solution and washing the plates, HRP-Streptavidin solution was added to each well and incubated at room temperature with gentle shaking.

After the indicated times, binding was terminated by removing the solution, and the cells were washed three times with 1 ml ice-cold ECF buffer.

King, originally from Goole in east Yorkshire, wanted to point out how absurd it was that, "in areas that have been wilfully run down and had their industry removed", the solution could be gargantuan art.

However the irradiation in the presence of catalyst completely removes the solution's antibacterial activity (AA).

Nitrogen was moderately flowed for 5 min to remove the solution and then it is covered.

Gel was transferred subsequently to vacuum filter system to remove the solution and subsequently washed with phosphate buffer.

Remove the solution and add rabbit anti-βgal primary antibody (MP Biomedicals, Solon, USA) diluted 1 20,000 in the same solution.