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A close approximation would involve direct quenching and extraction of the culture broth (i.e. cells + supernatants, therefore removing the problematic washing step) and a differential/subtractive method for estimation of intracellular metabolite concentrations [31].
We next undertook a detailed analysis of all the HITs where the aggregate Turker judgment differed from the gold standard, after removing the problematic mutations described above.
In the taxon-exclusion tests, after removing the "problematic" species/clades, the unrooted "backbone trees" were almost identical irrespective of the used methods (uMP and BI).
However, the use of the PLATON/SQUEEZE electron-counting feature is precluded, and removing the problematic reflections would sacrifice the requirement of data completeness, especially within the low-angle shells.
Removing the problematic fruit-browser class ("mabra" group, see methods section), did not affect the relative placement of the remaining species, and even P. ginsburgi fell in homologous clusters between the different data sets.
After removing the problematic markers from the ABH mapping data file in Microsoft Excel, the revised ABH mapping data file was used to construct the final linkage groups in MapManager QTX (Manly, Cudmore, and Meer 2001) with the following settings: linkage evaluation F2, search linkage criterion P = 0.05, map function Kosambi, and line cross cross-type.
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They made sure to ridicule it, and the bill may be sent back to remove the problematic points.
If politicians really want to make the laws dealing with social media fit for purpose, they must also remove the problematic language of "offence" from the Communications Act.
The House version goes further, allowing private companies to sue service providers for even briefly and unknowingly hosting content that infringes on copyright — a sharp change from current law, which protects the service providers from civil liability if they remove the problematic content immediately upon notification.
Her group's proposed strategy is to surgically remove the problematic blood vessels and replace the damaged RPE cells with new RPE cells derived from a patient's own cells.
The general protocol is to remove the problematic probes and group the remainder by the gene or transcript that they target.
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