To serve, remove the acetate-wrapped cake from the tin, then gently remove the acetate.
The resulting hydrolysis, or "alcoholysis," reaction removes the acetate groups from the PVAc molecules without disrupting their long-chain structure.
In that case, let the mounted leaves air-dry for 48 h. 9. Let cool and carefully remove the acetate.
The cells were loaded with the dye, 2',7'-dichlorofluorescein diacetate, after which cellular esterases removed the acetate groups and the dye was trapped intracellularly.
Here the two acetate groups render the molecule membrane permeant, while once inside cells, the active ester labels intracellular proteins, while esterases remove the acetate groups restoring the fluorescein's fluorescence.
When it is taken up by a living cell, esterases can remove the acetate groups and if it is oxidized (typically by peroxide), it is converted to fluorescent DCF.
Zemplén deprotection to remove the acetates and methyl ester was followed by acidic removal of the tert-butoxy groups and subsequent reduction of the azide using standard hydrogenation conditions yielded the monovalent compounds SC1 and SC2, which had an amine and a guanidine group at the four position of the sialic acid, respectively.
Finally, this emulsion was diluted six times with water and stirred at 800 rpm with a magnetic bar for 4 6 h to remove the ethyl acetate.
A solid liquid separation unit is needed to remove the sodium acetate in the liquid stream.
Remove the copper acetate from the heat before it completely evaporates, as it will quickly explode otherwise.
