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The clone-corrected analysis was performed by removing replicates of the same sequence type, as repetition of the same sequence type, due to clonality, can lead to the detection of linkage disequilibrium and consequently could affect the ability to detect recombination among genotypes.
After removing replicates, there were totally 10977 pairs of synthetic lethal interactions of 2640 proteins.
This involved sequentially processing the transcripts as follows: paraphrasing, investigating constructiveness and generalizability of comments, removing replicates, and bundling similar comments.
In the analyses of DMP and diploid family size, we included only those control crosses for which we determined ploidy level of all male offspring (i.e. N = 10 after removing replicates with <7 diploid offspring).
The same was true for the reserved test set which originally contained 213 data sets; this decreased to 160 when removing replicates and to 148 when data sets overlapping the training set were also removed.
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We compared the numbers of SNPs that depart from HWE in PBMC and LCL sample populations after filtering by population call rate and removing replicate samples.
Bring chamber of freeze-fracture machine to atmospheric pressure, remove replicated specimens with fine forceps and place under PBS in a well of a porcelain spotting dish.
Post hoc analysis of the ≥50% response threshold, which differed from the published result in that the treatment center effect was removed, replicated this finding (NASHA/Dx vs. sham treatment, 52.2% vs. 30.0% P=0.004).
Hence, the response detection rates did not change after removing the replicates with large variability.
In order to investigate if this was due to differences between our replicates we removed the replicate hybridizations with the best quality signal from the male dataset and repeated the calculations.
For each slide, we removed incongruent replicates calculating the ratio between ch1 and ch2 intensity for the 2 replicate with dye swap (respectively R1 and R2) and the standard deviation (SD).
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CEO of Professional Science Editing for Scientists @ prosciediting.com