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The CTC-iChip first separates nucleated cells from other blood components (i.e., "debulking" by removing plasma, platelets, RBCs, and free beads) by deterministic lateral displacement, before aligning nucleated cells in a single file by inertial focusing and magnetically deflecting bead-labeled leukocytes under continuous flow.
Free quinine was obtained by removing plasma proteins by ultrafiltration using Millipore Centrifree Centrifugal Filter Units.
One drop of blood obtained after removing plasma was applied to a microscope slide using a Pasteur pipette.
Erythrocytes were harvested immediately after removing plasma and white blood cells and stored at −20°C until assayed.
Only by completely removing plasma proteins from the cell culture and final formulation processes could the risk of infectious disease transmission from recombinant products be totally eliminated.
In our pilot experiments, we found that CD16 is not readily detectable in whole blood, whereas it is after removing plasma, probably due to the binding of CD16 by the Fc domain of antibodies present in the serum.
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Hemofiltration removes plasma water and soluble components below 25 kilodaltons.
Further, we have successfully developed a new plasma native peptidomic technology that allows to efficiently remove plasma high abundant proteins to comprehensively identify circulating native peptides using mass spectrometry9,10,36,37.
Those beads significantly removed plasma cytokines and chemokines and decreased BALF-to-blood chemokine ratios, resulting in decreased leukocytes recruitment into the lung.
This was repeated three times to remove plasma and buffy coat.
For erythrocytes, the blood samples were centrifuged at 1200 g during 10 min to remove plasma and buffy-coat.
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