From a physiological point of view, calcium currents cannot be blocked without also removing calcium-dependent potassium currents.
Morphometric measurements first were standardized by removing size-dependent variation per Elliott et al. [ 134], and then log-transformed.
This normalization removed intensity dependent biases from each printing block in each slide.
It can be noted that zero entries have been inserted to remove linearly dependent observations.
Loess normalization was applied to each array to remove intensity dependent trends [ 37].
Then lowess normalization, intra- and inter-slide normalization were applied to remove intensity dependent trends.
After filtering and background correction, all arrays were normalized using loess to remove intensity dependent variations in the ratios [ 41].
As shown by the figure, Loess normalization effectively removed dye dependent effects in the microarray and rendered evenly distributed ratios across all signal intensities.
For each replicate slide, the mean and background intensity values from each channel (W595, W685) were log2 transformed and normalized using the LOWESS algorithm to remove intensity dependent effects within the calculated values.
In this part of the text, we are referring to the fact that deletion of Snf1 partially inhibits the rapid dephosphorylation of Sch9 seen in glucose starvation conditions but removing the Snf1 dependent target sites in Kog1 does not.
