Exact(2)
We demonstrate that removing alignment errors due to 1) improper structure alignment, 2) the presence of paralogous sequences, and 3) partial or otherwise erroneous sequences, improves contact prediction by covariation analysis.
Pairwise alignments were used to construct reference sequenced anchored multiple read alignments by preserving alignment gaps in the sequence read but removing alignment gap-columns in the reference sequence.
Similar(58)
A total of 5886 ClustalW alignments, approximately equally representing these ncRNA families, were used for training after removing alignments that were not recognized as structured RNA by RNAz in both reading directions.
Manual inspection indicated that these discrepancies were due to GSNAP failing to remove alignment bias when aligning sequences to regions containing more than 5 and as many as 16 heterozygous sites.
Users are provided with parameters to disable the removal of redundant sequences and to remove alignment positions with a high fraction of gaps (insertions or deletions), considered to be of low statistical support.
Geneious was then used to create a consensus sequence for each individual, to align those consensus sequences, and to remove alignment columns containing ambiguous sites and gaps, respectively.
To remove alignment biases at heterozygous variants, AA-ALIGNER uses the allele-aware aligner GSNAP [ 24] which has been previously shown to remove mapping biases using complete genotype information [ 22].
For the purpose of phylogeny reconstruction (see below) the Gblocks program is widely used to remove alignment blocks suspect of low quality, according to various cutoffs on the number of gapped sequences in alignment columns.
Gblocks (version 0.91) [48] with the default settings was used to remove alignment regions that contain gaps or are highly divergent.
This latter was checked and edited by hand to remove alignment uncertainties.
The 3s program currently uses just one sequence per species at each locus, and it removes alignment gaps and ambiguous nucleotides from the alignment.
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