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After hatching, amoebae were incubated with 10 µg/ml blasticidin for 4 days to remove wild type cells and enrich for mutants.
When anti-HBs selection pressure is removed, wild-type HBV returns as the predominant infectious form because of the impeded replication of the Gly/Arg 145 mutant.
Selection through insecticide pressure will favor kdr alleles and remove wild-type alleles.
The isolated plasmid DNA library was digested with SphI and KpnI to excise the diversified dmsD genes, which were subsequently ligated into pDmsD-Y2 that had been similarly digested with SphI and KpnI to remove wild-type (wt) dmsD.
These results suggest that the C peptide is removed in wild type and kpc-1 but not in egl-3 mutants.
A 670 bp PstI/HindIII fragment from pPAHB Xa2FN1) containing the Factor Xa site codons was ligated into pYS5 similarly digested with PstI/HindIII to remove the wild type 670 bp fragment and the resulting plasmid was designated pYS1Xa2FN1 [ 22]. pYS1Xa2FN1 was transformed into Bacillus subtilis WB600 for expression of the PA/Factor Xa mutant [ 23].
We wondered if the DAPI-dense blocks in BAZ1B knockout cells could be removed by reintroducing wild type WSTF protein.
Fig. 6 shows the monotonic decrease in the T2 value where, at every stage, the least important feature is sequentially removed for each wild type vs. mutant pair.
Three EDL and three soleus muscles removed from wild-type lmna+/+, lmna−/+ and lmna−/− mice (n = 3 per age group) aged 4 weeks (mice still growing and active) and compared to those from 5 weeks old mice (mice no longer growing, often barely active).
Metal ions were therefore removed from wild-type ECAO using a modification of the method described previously for AGAO (31).
To reduce Pros expression, we either removed one wild-type copy of pros or knocked down Pros by RNAi in type II NB lineages.
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