The accessory reproductive organs were removed, weighed and processed for histopathological analysis.
At necropsy, organs were removed, weighed, and counted in the well counter (Wallac Wizard 1470, PerkinElmer, Waltham, MA, USA).
At time of sacrifice, necropsies were performed: the spleen, kidney, liver, and thymus were removed, weighed, and sent for histopathology.
These 4 muscles and gastrocnemius (GAST) then were removed, weighed, sectioned, and stained with adenosine triphosphatase for fiber typing and fiber area measures.
Mice were sacrificed after 3 weeks following the booster injection, the testis was removed, weighed and measured, and the histological structure was observed.
Immediately after d-xylose absorption test, all birds were killed humanely and the intestinal tract was removed, weighed and examined for gross lesions.
In selected animals, the lungs, liver, and heart were removed, weighed, and counted in the gamma well counter and then embedded in paraffin for sectioning.
Vegetations were exposed, photographed, aseptically removed, weighed, and homogenized in PBS for quantitative bacterial counts.
The brains were then removed, weighed and homogenized in 1.5 ml of PBS.
At sacrifice, a section of colon was removed, weighed, and immediately flash-frozen.
The lungs were then removed, weighed and dissected as indicated in Figure S1.
