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Oocytes before and after PBE were manually removed under the microscope.
The hippocampus, corpus callosum and the meninges were removed under the microscope.
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Tissue surrounding the trachea was removed under the dissecting microscope in HamF12 medium.
At 30 minutes to 1 hour after the injections, the skin and muscles above the spinal cord (including 7 10 muscle bundles caudal to the hind limbs) were removed under the dissection microscope.
The pericardial tissue was removed under a microscope and the LAD artery was ligated with 6 0 silk suture using a snare occluder [ 21].
The connective tissue was gently removed under a microscope and the ganglia were digested with a mixture of 0.4 mg/ml trypsin (Sigma) and 1.0 mg/ml A-type collagenase (Sigma) for 40 min at 37°C while agitated by gentle bubbling with 95% O2 and 5% CO2.
The connective tissue of the nerves was removed under a light microscope, and the nerves were then immediately placed in sterile distilled water and cut into 10-mm segments.
After washing in PBS, surrounding synovial tissue of the TMJ disc was removed under stereo microscope.
The mucosa was then removed under a dissection microscope and the submucosal plexus of Meissner (located directly under the mucosa) was carefully dissected with fine scissors.
Subcortical tissues and meninges were carefully removed under dissecting microscope.
The descending thoracic aorta was dissected free, and surrounding connective tissues and fat were removed under a microscope in a Krebs solution bath (118 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO4, 1.2 mM KH2PO4, 2.4 mM CaCl2, 25 mM NaHCO3, and 11 mM glucose).
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