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Brains from freshly slaughtered pigs are dissected and the choroid plexus tissue from the lateral and the fourth ventricles removed, transported with HBSS in Ca2+/Mg2+ (Gibco BRL, Eggstein, Germany) and HEPES buffer (BioWhittaker, Apen) to the laboratory, and treated hereafter with mixed cold and warm trypsinisation (0.2% solution, Biochrom, Berlin, Germany, 45 min. at 4°C, 20 min. at 37°C).
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The logic was that once customs duties are removed, transport costs will fall.
That means persuading neighbours to remove transport barriers.
The Right Reverend Crispian Hollis said removing transport subsidies could "severely impact the future and the flourishing of Christ the King School".
And he strongly criticised Prime Minister David Cameron's decision to remove transport secretary Justine Greening from her post, which he claimed was a prelude to a U-turn on a third runway.
Biopsy samples were manually disaggregated and aspirate samples centrifuged at 1200 rpm to remove transport medium prior to preparation of slides.
A 2008 memorandum on hold rooms and short-term custody states that "whenever possible, a detainee should not be held for more than 12 hours" and that "every effort will be made to promptly process, transfer, transport, remove, or release those in custody as appropriate and as operationally feasible".
The ripe fruit is removed and transported to the supermarket.
A continuous pipe is formed through the dam once appreciable aggregates of soil particles are removed and transported downstream by the flowing water.
During this maturation process, insulin is crystallized in the form of dense core, and unwanted cargoes and membrane proteins are removed and transported either back to the TGN or endo/lysosome systems (Kim et al. 2006).
They differ significantly from the angular coral rubble, which builds up the uppermost part of the barrier (Fig. 3) and was removed and transported onshore by a major hurricane (EWE XVI or later?) according to Scheffers (2005) and Scheffers et al. (2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com