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Hearts were rapidly removed, transferred into 10 ml 0.9% NaCl solution containing 1,000 U heparin, and connected to a cannula in a Langendorff perfusion system.
The pieces of each sample were removed, transferred to 2.5% glutaraldehyde in sodium cacodylate buffer 0.1 M for transmission electronic microscopy analyses or to Zamboni fixative solution [41] for light microscopy.
The supernatant was removed, transferred to a new centrifuge tube and re-centrifuged at 16,000 g for 10 min at 4°C to facilitate removal of the lipid layer overlying the supernatant.
The upper aqueous phase was then removed, transferred to a fresh tube, and 500 μL of isopropyl alcohol was added.
When the tumour size reached 100 mm, it was aseptically removed, transferred into ice-cold DMEM, and cut into 1 mm-sized fragments.
Trigeminal ganglia were removed, transferred into HBSS and enzyme-digested by incubation with papain (Worthington), collagenase type II (CLS2) (Worthington), and dispase type II (MB).
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5. Rinse with warm water and remove transfers (save them, they are reusable).
The ribosomal scaffold will tell us about the central tendency of the ribosome, after removing transfers between divergent organisms (such as described in [ 94] ) from the averaging.
This power grab resembles an organ transplant whereby the beating-heart of those oppressed is removed and transferred to this faux leader.
The rats' livers were then removed and transferred to clean beaker and weighed.
The supernatant containing cytosolic proteins was carefully removed and transferred to a new tube.
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