In the M-2 experiments, the ganglia were first treated with Protease (1 mg/ml) (Type XIV, SIGMA, USA) for 10 min at room temperature, washed out with saline solution, and the connective tissue sheath was completely removed to improve recording quality.
1 mL samples were removed periodically to record OD at 600 nm. 5 mL overnight cultures of E. coli pAC-4CL1 or pAC-4CL4 + pUCMod or pUC-STS were inoculated 1 100 into 50 mL modified M9 medium with glycerol containing chloramphenicol and carbenicillin.
"I have been on record for asking the president for corrupt officials not to be moved around but to be removed, to be neutralized; if records can prove conviction, to be arrested and convicted.
At regular intervals during the stress, the stress was temporarily removed in order to record transfer characteristics to monitor the degree of threshold voltage shift.
If this occurred, these records were removed to ensure that all sires were represented equally.
A threshold is then applied for both the median frequency and integral for each point to generate a 'mask' (i.e. a map of which audio is to be kept and which is to be removed) to apply to the audio recording.
Keenly interested in classical art, Elgin secured permission from the Turks after his arrival in Constantinople to record and remove Greek antiquities, fearing their destruction in the ongoing conflict between the Greeks and the Turks.
If both diseased and recovered/removed individuals are observable, then the epidemic sensitivity becomes high with an extended observation period, since individuals that are infected and recover or removed prior to recording are also observed.
The final major function of the 8515 was to remove recorded tracks.
The 20 recordings from each stimulus frequency and electrode depth were averaged, and then this average was low-pass filtered (100× decimation) to remove recording noise.
