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In contrast, large non-uniform microsphere distributions have been observed in surgically removed tissue samples after radioembolisation [14]-[17] [14]-[17]
Doctors removed tissue samples and sent them to Johns Hopkins University's neuropathology department in Baltimore.
The experiments we have conducted with surgically removed tissue samples represent a meaningful approximation to the natural processes of exposure and infection in the patient.
Freshly removed tissue samples were immediately fixed in 4% buffered formalin.
Freshly removed tissue samples were immediately fixed in paraformaldehyde solution for 12 24 h and paraffin embedded for immunohistochemical analysis.
Surgically removed tissue samples were snap-frozen in liquid nitrogen immediately after surgery and stored at −80°C until cryosection.
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Samples of removed tissue should be examined both histologically and microbiologically.
Gene expression profiles were obtained using cDNA microarrays for a total of 80 surgically removed pancreatic tissue samples (Table 1).
After 12 h, the animals were sacrificed, the pathogen loads in the cecum lumen, the mLN and the spleens were analyzed and we removed cecum tissue samples for histopathological analysis and for RNA extraction.
Paraffin was removed from tissue samples with xylene and was rehydrated through decreasing ethanol concentrations (100, 95, 80 and 70%).
Freshly removed pancreatic tissue samples were immediately fixed in 10% formalin for 24 hours and paraffin embedded.
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