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King, originally from Goole in east Yorkshire, wanted to point out how absurd it was that, "in areas that have been wilfully run down and had their industry removed", the solution could be gargantuan art.
We removed the solution into 96-well plates carefully, and the sample was freeze-dried at room temperature.
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However the irradiation in the presence of catalyst completely removes the solution's antibacterial activity (AA).
Nitrogen was moderately flowed for 5 min to remove the solution and then it is covered.
Gel was transferred subsequently to vacuum filter system to remove the solution and subsequently washed with phosphate buffer.
After removing the solution, 30 μL of 50 mM ammonium bicarbonate was added.
Remove the solution and add rabbit anti-βgal primary antibody (MP Biomedicals, Solon, USA) diluted 1 20,000 in the same solution.
After removing the solution, the gel spots were incubated with 200 mM ammonium bicarbonate for 20 min.
After the indicated times, binding was terminated by removing the solution, and the cells were washed three times with 1 ml ice-cold ECF buffer.
After removing the solution and washing the plates, HRP-Streptavidin solution was added to each well and incubated at room temperature with gentle shaking.
After removing the solution, the disks were washed with water and ethanol, and incubated for 15 min in a solution containing 100 mmol L−1 CDI in acetone.
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CEO of Professional Science Editing for Scientists @ prosciediting.com