Exact(3)
Site-specific recombination in vivo removed the selection cassette, leaving behind the Mu R-ends and one loxP site; and this was confirmed by sequencing.
Once variation is removed, the selection to retain imprinting is lost.
Moreover, with the help of the Cre-LoxP system we removed the selection marker gene which is absolutely nonessential for expression of target proteins.
Similar(57)
Clicking once on the check box selects it; clicking again removes the selection.
This plasmid was stably maintained in the 01-B526 trassformants, as were the native plasmids, but could be easily cured by removing the selection conditions.
The department is also said to be angry at the subsequent actions of the committee, which, like the 10 other panels set up around the country for the first time this year in an attempt to remove the selection of circuit court judges from the political process, was to have advanced the names of five possible contenders and left the final decision up to the Justice Department and the White House.
The database system rewrites the query by replacing the original table name with the identified fragment name and removes the selection condition containing a for the relaxation attribute.
More unexpectedly, this should also lower the computational cost because it removes the selection process and, above all, because in the case of lines and circles, the voting is a one-to-one projection from the image to the parameter space.
To prevent possible detrimental effects of the selection cassette, Rad1K185R mice were crossed with the Flpe deleter strain to remove the selection cassette in vivo [30].
This would disrupt the interaction between mothers and her offspring and therefore remove the selection pressure on imprinted expression.
After induction of I-SceI expression by anhydrotetracycline, only a small proportion of cells should survive; these cells have healed the double strand break introduced by I-SceI by a homologous recombination that removes the selection cassette.
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