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The poultice was then removed, the sample rinsed with water and dried in air.
To examine the bactericidal effect of the sample indirectly, we have removed the sample on the MH agar and further incubated the agar for 24 h to observe if there was any bacteria growth on the open area once occupied by the samples.
"I'm in It" began with a different sample and melody, but West removed the sample and Rubin edited the track down from a six-minute arrangement.
After the retina was removed, the sample was transferred to phosphate-buffered saline (Life Technologies, 10010, pH 7.4, Ca2+- and Mg2+-free) and cut radially into 4 to 6 pieces from the optic nerve head to the periphery.
After 10 min incubation at room temperature the samples were centrifuged at 4500× g, the supernatant was removed, the sample was snap-frozen in liquid nitrogen and finally stored at -80°C.
In order to avoid suboptimal fixation of surgical specimens and resulting problem of loss of immunoreactivity of phosphoproteins in paraffin samples, surgical samples were processed as follows: tissue samples were moved from the operating room to the pathology room as soon as surgeons removed the sample from the patient.
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Once these hard parts were removed, the samples were drained on a 0.5 mm sieve, blotted dry and the total mass of the sample was taken.
We removed the samples with any known genetic risk factors and assessed the impact of c.180C>T within different etiologies.
After the scales were removed, the samples were rinsed with 0.5% potassium hydroxide (KOH) solution and stained with ethanol-saturated 20% alizarin red S/0.5% KOH for 1 2 days.
If you have any doubt about the acceptability of the sample size, remove the sample buffer from the AHDC sample tubes and submit the sample in the dry tube.
Furthermore, the described procedure allows external thermal sample pretreatment with preceding pyrolysis and additional vaporization, condensation, and re-precipitation that significantly reduces or removes the sample matrix.
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