In addition, we removed strains that are the result of specific manipulations of other strains in the laboratory and genomes that represent multiple clones taken from a single site rather than different strains.
(D ) Western blots of SpoIIE compartmentalization mutants with TagSpoIIE removed (strains RL5911-5916, with strain RL5876 as a reference) as in panel C. DOI: http://dx.doi.org/10.7554/eLife.08145.010 10.7554/eLiFigure4 sourceigure 4—source data 1.
Cool ribs in liquid for 1 hour, then remove; strain liquid.
Other ways to remove strain?
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To remove strain from the docked poses, the systems were put through an energy minimisation procedure using the Impact module of the Schroedinger software.
This latter capability is important, as it allows for mutagenesis screens on homogenous genetic backgrounds, removing strain-specific phenotypic variation as a confounder.
Armed with these two lists, you are in a crucial position to make decisions about how to remove stress, reduce strain, increase the energy-increasing activities in your life, and find more moments of pleasure throughout the day.
This analysis removed both strains from the Bormi region, and left four strains found in the Charmaguria, Dhamrai, Dokhalo and Karamjal monkeys, which were identified as non-recombinant.
Any remaining pieces of tissue were removed by straining the solution through a nytex filter, and the cells in suspension were centrifuged and resuspended in DMEM + 5% FCS.
