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The supernatant fraction was removed, saved and the pellet extracted again using lysis buffer plus 1.0% SDS.
Prior to transfection, medium was removed, saved, and replaced with 500 µl Optimem (Life Technologies, Great Island, NY) and incubated for 30 60 min. 105 µl of transfection buffer (274 mM NaCl, 10 mM KCl, 1.4 mM Na2HPO4, 15 mM glucose, 42 mM HEPES, pH 7.06) was added drop-wise to a 105 µl solution containing 7 µg of DNA and 250 mM CaCl2, with gentle vortexing.
Similar(10)
And that's the satisfying denouement of the tale in its often-told, bare-bones form: John Snow pioneers the science of epidemiology and, by having a pump handle removed, saves hundreds of lives.
The unstructured SMC grid allows time step to be relaxed and land cells to be removed, saving over 1/3 of the total computation time in comparison with the original latitude longitude grid model.
A malignant melanoma was diagnosed and removed, saving the woman's life.
Egg-specific steps in the production process would be removed, saving time.
Animals were anesthetized using ketamine and xylazine and the adrenal glands removed, save for one group which received a sham surgery.
It had no licence plates and the interior had been removed save for the back seat.
It had no license plates, and the interior had been removed save for the back seat.
The original linings were removed and saved, the tapestries were painstakingly washed, and the linen rectangles bearing the marks and stamps were attached to the new backing straps on the tapestries, under the new cotton sateen linings.
For staining, the gel was immersed in coomassie staining solution and rocked for 30 min, and then, the stain was removed and saved for future use.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com