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Surgically removed samples were immediately frozen in liquid nitrogen and stored at -80°C until use.
Surgically removed samples were fixed in 10% formalin and embedded in paraffin.
After the middle lobe of the right lung was removed, samples were fixed in paraformaldehyde for 48 h and embedded in paraffin for histological studies.
All removed samples were placed in saline solution and sent to the lab for cryofixation in ethane solution within 1 hour.
The block was removed, samples were washed in PBTw, primary antibodies were added, and samples were put at 4° for 16 20 hr.
The supernatant was removed, samples were washed three times in PBTw for 5 min each, and secondary antibodies were added for 2 hr at room temperature in the dark.
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After the surgical glove was removed, sampling was performed as for the immediate effect.
The Cu-removed samples were transferred to HCl 10% solution, held for 5 min to remove contaminants from samples, and then transferred to DI water for 10 min to remove any residuals.
After SOX, NOX, and halide species were removed, sample CO2 was converted to graphite via catalytic reduction of CO.
Plant residue and gravel were removed and samples were mixed uniformly to form one composite sample.
After 24 h, frames were removed and samples were placed in mixture of water and some lime for 27 days.
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CEO of Professional Science Editing for Scientists @ prosciediting.com