Once the mRNA poly(A) tail is removed, reverse transcription reaction will not proceed, resulting in low concentrations of truncated transcripts (see Figure 1b).
A trimming database was used to remove reverse transcription adapters (from SMARTer kit) from the sequencing reads prior to assembly.
In contrast, in order to maintain the scale sensitivity other researchers suggested instead of removing reverse-worded items these items be reworded.
Importantly, site-specific mutagenesis experiments that either removed or reversed the charges at specific residues within this proximal CTD resulted in a decrease in tetramer assembly.
The proportion of variation captured by PC1, which represents the taurine indicine split, decreased with increased gene flow in the unbiased ascertainment treatments, whereas this relationship was removed or reversed in the biased treatments (Additional file 1: Table S5).
By comparing networks that differ only by a single directed edge, either added, removed, or reversed, a GHC method can estimate networks of larger scale than a search of all possible networks and do so on a workstation rather than a supercomputer, thus overcoming two problems at once.
Furthermore, chimeric DNA/RNA primers need to be removed and reverse-transcribed in order for the splicing to be completed.
Unlike other surgeons who predicted 12 to 18 months of recovery, he told me that he would remove and reverse the colostomy as soon as possible.
The space of possible networks was explored using the TabuSearch algorithm and, at each iteration, the score was evaluated by adding, removing or reversing individual edges.
However, even after a robust ORE has emerged in childhood, the face-processing system remains flexible so that the ORE can be removed or even reversed if there is sufficient exposure to other-race faces [10], [11].
