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Lungs were then removed, quick frozen in liquid nitrogen and stored at −80°C until nuclear protein was isolated.
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Around Barnbrook, officials – joyous at having the stain on the authority so ruthlessly removed – were quick to share the good news with friends and loved ones.
The excess of fluid was removed by quick wicking on a gauze pad prior to weighing.
Animals were then sacrificed on day 4 and 14 post-viral injection, their liver tissues removed and quick frozen on dry ice, and stored at –80°C until use.
Unincorporated nucleotides were removed by Quick Spin columns (Boehringer Mannheim, USA).
A light haematoxylin counterstain was performed and the haematoxylin was then removed by quick dip in acid alcohol and the slides were washed in ammonia water, dehydrated and mounted with Permount.
Adult animals were sacrificed on day of life P120, their brains were removed and quick-frozen.
The organs of interest (i.e. brain, heart and testis) were removed and quick-frozen in i-pentan.
Brains were carefully removed and quick-frozen in liquid nitrogen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com