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We initially evaluated the base quality of the sequenced reads (Additional file 1) and trimmed poor quality bases as well as removed poor quality reads (Additional file 2).
To get high-quality small-RNA (sRNA) reads, we removed poor quality reads and adaptor sequences (5'-ATCTCGTATGCCGTCTTCTGCTTG-3') using the FASTX toolkit (downloaded from http://hannonlab.cshl.edu/fastx_toolkit/download.html, [ 82]), from raw data (Table 3).
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Two, the recent crackdown on counterfeit medications may have been successful in removing poor quality medications from the markets.
The data set was adjusted slightly to use a selection of stations more evenly distributed azimuthally, while removing poor quality waveforms.
Chromatograms were viewed in ChromasPro version 1.34 software and edited to remove poor quality sequences at each end; the sequence was then saved in FASTA format.
Fog computing is also being used as a solution for food traceability management, where the aim is to remove poor quality products from the supply chain using value-based processing [72].
To maximize the accuracy of bacterial identification, we rigorously screened sequences to remove poor quality reads.
After removing poor quality sequences and duplicated copies, we obtained 696 ESTs that were only expressed after the pathogen attack.
We removed reads containing ambiguous bases and of read lengths <100 nucleotides, separated those derived from + and – strands, and manually trimmed trailing ends to remove poor quality data.
Imagene's automated spot flagging feature was used to identify and remove poor quality spots (i.e. those contaminated by small dust particles).
All three data sets were rigorously screened to remove poor quality reads (short reads <200 nt, zero-ambiguous sequences, and homopolymers <6) and to ensure that sequences were aligned over the same range of starting and ending nucleotides.
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CEO of Professional Science Editing for Scientists @ prosciediting.com