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Ten cm plates of cells (cultured for 2 passages without drug to 80% confluence) were rinsed twice in Dulbecco's PBS, removed of excess liquid using a pipette tip, and placed on ice.
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The entire trachea was removed, cleaned of excess connective tissue, and opened longitudinally via a midline incision along the ventral surface.
Heads were then removed, trimmed of excess tissue, post-fixed for 24 hours and decalcified in RDO (Apex Engineering Products; Aurora, IL, USA).
Defects exceeding the project criteria are thoroughly removed; however, the removal of excess material should be minimized to minimize local stresses.
Tracks are removing thousands of excess seats and sponsors are harder to find.
The purification step was estimated to remove >95% of excess myosin motors.
Try to remove areas of excess red or blue around the edges.
Such support provides extended areas for cells to grow and spread, with the possibility of removing excess of medium by touching the filter paper at the back-side of the grid, without disturbing the structure of the cells.
Gently washing of the endothelial cells after RGDPEG-avidin incubation removed the excess of unbound RGDPEG-avidin, leaving only cell-associated avidin after incubation at room termperature.
An experienced technician weighed the CL three times, after soaking the CL, and removed the excess of liquid.
Exposure of the acid leached catalyst prepared at 1000°C to Cl2 at 650°C removed additional quantities of excess iron and increased the catalyst activity even further.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com