Whole brains were removed from mice naive to any treatment, quickly frozen on dry ice and stored at −80 °C.
To determine whether the presence of chemical residues on pupal cases might account for the observed changes in adult behavior, we applied EA odors to the pupae of naive larvae and removed any residual EA odors from EA-conditioned larvae by washing their pupae.
DOI: http://dx.doi.org/10.7554/eLife.07423.004 To test whether the decrease in oviposition can be transmitted from students to a new batch of naive flies, we removed Canton-S pre-exposed teacher females from student His-GFP expressing flies and placed the teacher-instructed student flies in a new chamber with 3 new, naive Canton-S flies.
DRG were surgically removed (L4 and L5 in naive animals; L5 in nerve-injured animals), enzymatically treated, mechanically dissociated, plated on glass coverslips previously coated by a solution of 0.1 mg/ml poly-D-lysine, and incubated in MEM-BS at 37°C, 3% CO2, and 90% humidity for 2 hr.
Wasps were then removed and replaced with 3 female, naive student flies of the opposite genotype (either His-GFP or Canton-S depending on teacher identity).
Following wasp exposure, we surgically removed fly heads and paired them with naive student flies.
It was an incredibly naive and somewhat selfish dream which removed anything she was going through emotionally and physically from the scenario.
To isolate memory T cells, naive CD4 + T lymphocytes were removed by negative selection using anti-CD45RA (eBioscience) and depleted by incubation with Dynabeads Pan Mouse IgG (Invitrogen).
We made a ϕ24B lysogen of strain TUV93-0 (EDL933 with both Shiga-toxigenic phages removed) and compared the acid resistances of the naive and lysogen strains.
Therefore, it might be possible that acute cytisine does not lower ICSS thresholds in nicotine-naive rats because it is rapidly removed from the brain.
