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The removed movements are those classified as 'No-contact', as there are no domain contacts in either conformation.
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Data were analyzed after removing movement or eye blink artifacts.
Voxel time series for each session were then pre-processed to remove movement-related variations of no interest and very low-frequency drifts by regressing on the previously estimated motion parameters and 0th 4th order Legendre polynomials as predictors.
After sampling, the signal will be high-pass filtered at 20 Hz to remove movement artefacts.
Functional volumes from each subject were spatially realigned and unwarped to remove movement-related signal intensity changes.
The six head motion parameters, as assessed by the realignment algorithm, were treated as covariates to remove movement-related variance from the image time series.
After the measurement, the raw laser Doppler signals were first filtered using a special custom-made digital filter to remove movement artefacts caused mostly by respiration of the mice (see Figure 5A as an example), thus resulting in a smoothed and downsampled (1 Hz) version of the raw signal, which represented the actual baseline blood flow level.
A catheter for tracheal tube exchange proposed by the extubation guidelines of the Difficult Airway Society DASS) was placed, endoscopic examination was performed while inducing spontaneous breathing and swallowing reflex by an otolaryngologist, and the tube was removed while movement of the tissue around the glottis was visually evaluated.
Sometimes the motion smoothing process removes real movements and/or introduces a delay in them.
Twenty-four regressors were computed based on an expansion of the movement parameters estimated during realignment and included as nuisance covariates in the design matrix to remove residual movements artefacts with spin history effects.
In these analyses, realignment parameters, as well as eye movement traces, were included as covariates of no interest to remove eye movement and brain motion artifacts.
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