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Expert is a match of the original notes; hard has some note-heavy drum rolls and other difficult or fast notes removed; medium takes away the orange notes (for guitar and bass) and anything else considered tricky.
Removed medium was replaced with sterile COMBO medium.
Removed medium was frozen and stored at −80 °C.
Complexes were removed, medium replaced and cells left for 24 h.
The removed medium proved cytotoxic to nonirradiated cells, indicating that irradiation per se was not responsible.
Removed medium and formazan crystals were solubilized by adding 150 μL DMSO.
Similar(52)
Suspensions (1 mL) were washed by deionized water to remove medium on the mineral surface.
After removing medium, 0.318 mM 4-MUNANA (2′- 4-methylumbelliferyl -α-D-N-acetylneuraminic acid; Biosynth Intl).
After removing medium, cells were fixed in 4% buffered paraformaldehyde (pH 7.4) for 30 min. Slides were rinsed twice in PBS, pH 7.4.
Remove medium.
Remove medium and wash with PBS twice.
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CEO of Professional Science Editing for Scientists @ prosciediting.com