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During the scratching, the AFM probe removed matrix thiol 1 and produced bare gold squares exposed to thiol 2 (COOH-terminated) solution (nanoshaving) [13].
In an attempt to create more porous structures for cell repopulation, we selectively removed matrix components from decellularized porcine aorta to obtain two types of scaffolds, namely elastin and collagen scaffolds.
The resulting suspension was subjected to mild centrifugation (100 g for 10 minutes) to separate the cells and their associated matrix in the pellet (cell-associated matrix (CM) compartment) from molecules derived from the matrix further removed from the cell surface in the supernatant (further removed matrix (FRM) compartment) as described previously [ 25].
The objective of this study was to determine how initial chondrocyte density in an alginate hydrogel affects the matrix composition, its distribution between the cell-associated (CM) and further removed matrix (FRM) fractions, and the tensile mechanical properties of the developing engineered cartilage.
Similar(56)
Capacity PNSRL removes matrix calculations to simplify large calculations.
Sample preparation usually requires solid phase extraction to remove matrix components and enrich the analyte.
Off-line SPE is one of the most common methods used to concentrate analytes and remove matrix interferences to achieve the desired levels of analytical sensitivity [26, 27].
To effectively remove matrix interfering substances from field water samples before instrumental analysis with HPLC/MS/MS, a multi-sorbent solid phase extraction method was developed using Box-Behnken design.
Following MSI the tissue sections were washed in 70% ethanol for five minutes to remove matrix.
First, we remove any prior knowledge on GO term relations (i.e. we remove matrix L 2 ).
Specimens were prepared by using water to disaggregate the matrix and steel periodontal probes and bin angled chisels to remove matrix from the cuticle.
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